Agilent Technologies, Inc. - Diagnostics and Genomics Pfu DNA Ligase, 400U
Pfu DNA Ligase, 400 U, from Agilent Technologies, is a thermostable DNA ligase derived from Pyrococcus furiosus. This enzyme is designed for high-fidelity ligation of DNA at elevated temperatures, making it suitable for applications such as ligase chain reaction (LCR), site-directed mutagenesis, and other molecular biology workflows requiring precise DNA joining. The thermostability of Pfu DNA Ligase allows for efficient ligation of nicks in double-stranded DNA at temperatures up to 70°C, reducing non-specific ligation and improving specificity in demanding protocols.